HPV Genotyping in Invasive Cervical Cancer Samples from Women Attended at an Oncological Hospital between 2017-2021 in Ecuador

Author(s)

García Muentes GD1, Abdul F2, Beltran C3, Medina S3, Ruiz Cabezas JC1
1Sociedad de Lucha Contra el Cancer (SOLCA) Matriz - Guayaquil, Guayaquil, Guayaquil, Ecuador, 2MSD, Bogota, Colombia, 3MSD, Bogota, CUN, Colombia

OBJECTIVES: Human papillomavirus (HPV) infection is a significant risk factor for the development of cervical cancer. Understanding the prevalence of HPV genotypes is crucial for designing effective prevention strategies. This study aimed to determine the distribution of HPV genotypes in invasive cervical cancer samples from Ecuadorian women who attended the Cancer Institute (Sociedad de Lucha Contra el Cáncer- SOLCA).

METHODS: Archived formalin-fixed cervical cancer tissue samples collected between 2017-2021 were deparaffinized, and nucleic acid extraction and purification were performed using silica columns. The obtained nucleic acids were analyzed using INNO LIPA HPV Genotyping extra II per the manufacturer's specifications. Cases were identified from the hospital-based cancer registry using the ICD-10 code for cervical cancer. Based on the estimated incidence of cervical cancer in 2018, according to the IARC a minimum of 70 invasive cervical cancer samples were estimated to achieve a 5% precision level. Exclusion criteria included low-quality or non-confirmatory tissue samples. Data was retrieved from records, and HPV genotypes were determined from the formalin-fixed paraffin-embedded (FFPE) samples.

RESULTS: The study included samples from 190women diagnosed with invasive cervical cancer, with a median age of 52 years. Squamous cell carcinomas accounted for 78.94% of the cases, while 21.05% had adenocarcinoma. The samples were obtained from women with a low socioeconomic status (81.6%) and elementary-level education (53.7%). Among the 190 samples, 80.52% tested positive for HPV DNA, while 19.47% were negative. The most common genotypes detected were 16 (64.05%), 18 (16.99%), and 58 (6.54%). Multiple genotypes were detected in 26 samples. HPV infection frequency was significantly higher in samples from patients with an elementary level of education (p < 0.05,).

CONCLUSIONS: This study provides valuable insights into the distribution of HPV genotypes in cervical invasive cancer samples from Ecuadorian women. The results indicate an elevated presence of HPV16, HPV18, and HPV58, being these types vaccine-preventable.

Conference/Value in Health Info

2024-05, ISPOR 2024, Atlanta, GA, USA

Value in Health, Volume 27, Issue 6, S1 (June 2024)

Code

EPH231

Topic

Epidemiology & Public Health

Topic Subcategory

Public Health

Disease

Reproductive & Sexual Health, Vaccines

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