OBJECTIVES: Registered nurse (RN) burnout is associated with poor patient quality outcomes such as increased mortality, high hospital readmission rates, and increased hospital-acquired infections. Catheter-associated urinary tract infections (CAUTIs) are a type of patient hospital-acquired infection associated with inadequate RN sterile urinary catheter placement techniques and/or a lack of frequent RN catheter care maintenance. RN burnout is associated with increased patient CAUTI rates given that RNs experiencing burnout exhibit decreased concentration, decreased time to complete patient tasks, and increased workload burdens. Given high RN burnout prevalence rates ranging from 34% to 50% across healthcare settings in the United States, high patient CAUTI rates associated with RN care may drive unnecessary medical overuse and increased health care spending. We assessed the cost of RN burnout versus non-burnout-attributed delivery of CAUTI patient care using a decision tree analysis. METHODS: A decision tree was created to project costs from a health care organization (hospital) payer perspective over 10 years. The incremental cost of CAUTI patient care delivery was compared in two scenarios, patient care provision by RNs not experiencing burnout (no burnout) and care provision by RNs experiencing burnout. Model inputs were informed by a systematic nursing literature review and AHRQ’s Healthcare Associated Infections Costs Report. Utilities were assigned to the RN burnout scenario in order to capture incremental costs per CAUTI occurrence. RESULTS: Over 10 years, the incremental cost effectiveness ratio (ICER) of RN-burnout attributed versus non-RN burnout attributed CAUTI care was $4,493. The incremental cost of RN burnout-attributed CAUTI care was $315 per 0.07 CAUTI occurrence. CONCLUSIONS: The incremental cost of CAUTI care demonstrates that RN burnout has detrimental patient quality and hospital cost outcomes. Developing strategies to address RN burnout may contribute to improved patient quality outcomes and healthcare cost savings.