OBJECTIVES: To confirm, in everyday practice, results from randomized clinical trials indicating that DuoTrav (a fixed travoprost and timolol combination) controls intra-ocular pressure (IOP) better than Xalacom (a fixed latanoprost and timolol combination), even when measured >24 hours after last instillation. METHODS: Patients with ocular hypertension or primary open angle glaucoma and treated by one of the above combinations were included in this cross-sectional study. Demographics, medical history and previous treatments were abstracted from medical records. IOP and treatment time were collected during an office visit. Analyses of variance, logistic regressions and propensity scores were used to adjust for confounding factors. RESULTS: In total, 328 patients were included, 127 treated with DuoTrav and 201 with Xalacom. The mean age was 64.6 years and 51.5% were female. Most (275: 84.6%) had last instilled treatment the previous day. Treatment groups were comparable except that Xalacom-treated patients had longer disease and treatment durations. Overall mean IOPs were 24.9 mmHg at diagnosis and 21.1 mmHg upon starting the fixed combination treatment. There was no significant difference between the groups as they started their second line therapy. Duotrav-treated patients experienced better IOP control (17.1 versus 19.1 mmHg: p<0.001). A difference was also noted for patients who missed their last scheduled treatment (17.2 versus 20.1 mmHg: p<0.006). Better IOP control with DuoTrav was further supported by patients whose last instillation was 9.00-12.00 hours before IOP measurement (16.5 versus 19.3 mmHg; p<0.001). According to the practitioners, 83.1% of the DuoTrav-treated patients attained their IOP targets, as compared to 51.3% of Xalacom-treated patients (p<0.001). All these differences persisted after adjustment for confounding factors. CONCLUSION: This everyday practice study paralled the published corresponding prostaglandin results of Topouzis and DuBiner, i.e. compared to Xalacom, IOP control with DuoTrav is better and has a longer residual effect when measured >24 hours later.