OBJECTIVES: Considerable evidence of the positive impact of pharmaceutical care services on patient's HRQoL outcomes has been reported. However, the implementation of HRQoL assessment in routine pharmaceutical care has been limited. This study aimed to assess the relevance of and challenges in the application of HRQoL evaluation in the provision of pharmaceutical care. METHODS: A self-administered survey of pharmacist was conducted during a regional continuing education program in New York metropolitan area. 83 pharmacists working mainly in community setting participated in the study. RESULTS: Majority of the respondents were male (81.9%), with extended (>16 years) practice experience (65.1%). More than 78.3% of respondents indicated that HRQoL measures could be a useful tool to assess patient's health status. 55% of the respondents agreed that positive results of HRQoL evaluations could help in obtaining reimbursement for pharmaceutical care services. However, about 70% reported no familiarity with instruments such as SF-36/12, EuroQOL, or FACT. Respondents rated clinical activities such as patient outcomes evaluation and designing a therapeutic monitoring plan as less important in the provision of pharmaceutical care compared to conventional activities (p <0.05). Those who reported better understanding about pharmaceutical care tend to report greater knowledge regarding HRQoL outcomes assessment. (p <0.05) More than half reported difficulty in choosing appropriate HRQoL instruments (56.6%) and challenges in interpreting HRQoL results (54.5%) as major barriers. About 84.6% of the respondents indicated willingness to learn more about HRQoL assessment to address such issues. CONCLUSIONS: Study respondents recognized the positive implications of HRQoL evaluations in practice. Incorporating HRQoL measures in practice could facilitate better understanding of the focus of pharmaceutical care among practitioners. However, further training and education is required by a pharmacist to implement HRQoL evaluation. Further research to include the perception of pharmacists from different practice settings and recent PharmD graduates is warranted.