OBJECTIVES: To evaluate the psychometric properties of MINI-HIV in HIV patients in Spanish population.METHODS: Validity, reliability and responsiveness of MINI-HIV were assessed throughout observational, longitudinal and multicentric study with 3 months of follow up. The 206 patients with HIV included in the study were divided into two groups: 50 patients within the reliability group (patients who were not foreseen any clinical change in the following 15 days) and 156 patients within the responsiveness group (patients that started a treatment or had a treatment). Thus, 46 blood donor subjects were to include within the control group. The main variable in the study was MINI-HIV (17 items). Other variables analysed on the study were; diagnosis date, disease symptoms, stage, previous HAART and actual HAART, MOS-HIV and general health status. RESULTS: The mean (SD) age of the participants was 37 (10.2), with a predominance of men (83.5 %). A total of 27.6% of the responsiveness group were ‘naïve' patients. 53.8% of the responsiveness group and 68% reliability group patients took only one HAART in the last year. 90% of patients answered all questionnaire items. The mean time(SD) of questionnaire filling by the patients was 12 (15.3) minutes. Regarding validity, MINI-HIV scoring showed a significant relationship with scoring assessed through MOS-HIV (R= -0.71; P<0.01), and through general health status (p<0.01 ). MINI-HIV scoring showed a significant relationship with CD4 (p<0.01), viral load and symptoms (p<0.001). Regarding responsiveness to change, a high effect size in MINI-HIV was observed between those patients whose general health status had improved during the follow up visits (ES=0.694 IC 95% 0.134-1.254). The internal consistency of the questionnaire was assessed through the a Cronbach (a= 0.93). The ICC (test – retest reliability) was 0.86. CONCLUSIONS: The MINI-HIV has good psychometric properties and correlated with clinical markers of the disease.