OBJECTIVES: Quinolones are commonly prescribed antibiotic in our hospitals and studies indicated their irrational use. This study analyzed the quinolones utilization in outpatient clinics of two teaching hospitals in Nigeria to generate information for promoting the rational use of quinolones. METHODS: A retrospective cross-sectional survey of two hospitals from 2013 to 2015, based on WHO recommended drug use indicators was conducted. Data for quinolones use was collected from prescriptions and patients medical records covering the period under review using validated forms. Data was analyzed for drug use indicators, dosage forms encountered, co-prescribed antibiotics, duration, distribution, and adherence to generic prescription. RESULTS: A total of 480 prescriptions were systematically selected from the two hospitals. The mean distribution of quinolone use for males was 192(39.3%) and 288(60.7%) for female. The mean age distribution for use was 1-12 years 10(1.9%), 13-24 years was 79(16.0%) while 25≤ years was 391(81.5%). The mean number of antibiotics per prescription was 2±2.5. The percentage of antibiotic per prescription was 33.4%. A total of 11 antibiotic classes were recorded with mean quinolones composition of 21.2%. Ciprofloxacin 279(59.85%) was the highest prescribed quinolone followed by ofloxacin 104(20.5%) while gemifloxacin 3(0.5%) was the least prescribed. Tablets were the highest prescribed dosage form 475(99.0 %) followed by parenteral 4(0.83) and otic 1(0.17%). Highest quinolone combination was between ciprofloxacin and metrnidazole 43(32.5%). The proportion of quinolones prescribed by generic was 258(56.3) while mean duration of quinolone use was 9±3.4 days. CONCLUSIONS: There is limited information on quinolones use in developing countries. This study indicated non-adherence to generic prescription, which can lead to increase in cost of medicines and non-adherence with consequent treatment failure, development of resistance and complications. Study suggests rational use of quinolones in the exception of non-generic prescription.