OBJECTIVES: Antibiotics' and injectable medicines' over-prescribing is common in ambulatory care in many low- and middle-income countries. We evaluated the effects of regulations in two different contract settings in SSO on physicians prescribing behavior. METHODS: All claim data of SSO in Tehran city were collected to evaluate the WHO indicators relevant to common concerns over physician prescribing. The indicators were selected more objectively and included: (i) average medicine items per prescription; (ii) average price per prescription; (iii) proportion of prescriptions including at least one injectable medicine item; (iv) proportion of prescriptions including at least one antibiotic medicine item; and (v) proportion of prescriptions including at least one corticosteroid medicine item. Four tests have done include Wilcoxon, Man-Whitney, Kruskal-Walis and Spearman by IBM SPSS Statistics 22 for Windows to analyze data. (Distribution of data wasn't normal, (pvalue≤0.05)). RESULTS: