OBJECTIVES: The measurement of health-state utility values (HSUVs) for a representative sample of Australian people with Multiple Sclerosis (MS) has not previously been performed. The purpose of this study was to estimate the HSUVs in Australian people with MS at different levels of disease severity. METHODS: HSUVs were calculated by mapping five questions from the WHOQOL-100 to the EQ-5D-3L descriptive system, then applying Australian general population-based utility weights to each level in each dimension. A multiple linear regression was used to investigate the relationship between HSUVs and disease severity, classified as mild (Expanded Disability Status Score–EDSS levels 1–3), moderate (EDSS levels 4–6) and severe (EDSS levels 6.5–9). RESULTS: Mean (95% CI) HSUV for all people with MS was 0.66 (0.65-0.67).  HSUV decreased with increasing severity of MS: 0.78 (0.77-0.80), 0.61 (0.60-0.62) and 0.48 (0.45-0.50) for mild, moderate and severe MS respectively. Adjusted differences in mean HSUV between the three severity groups were significant (p<0.05). CONCLUSIONS: This study is the first to quantify the impact of increasing severity of MS on HSUV of people with MS in Australia. The study has demonstrated that increasing MS severity considerably impacts the HSUV of people with MS. The findings of this study will be useful in the cost utility analysis of various interventions that slow MS progression.