OBJECTIVES::  to identify patients with potential indication to OPAT among inpatients with antimicrobials at a Brazilian public tertiary hospital. METHODS::  a point prevalence cross-sectional study was performed at August, 2016, before the implementation of cost-effectiveness OPAT project. All patients using parenteral antimicrobial were evaluated for a multidisciplinary team composed by pharmacist, nurses, physicians and social workers to evaluate pre-requirements for a safety transitional care to OPAT (stable clinical condition, venous access, social and logistic issues). Pediatric patients or with critical conditions or surgical antibiotic prophylaxis or with oral antimicrobial alternative were excluded. RESULTS::  Among 390 inpatients, 192 (49.2%) were in antimicrobial therapy, 59.4% were male with median age of 52 years. Piperacillin plus tazobactam, meropenem, ciprofloxacin, vancomycin and B polymyxin were the antimicrobials more frequently used. Antimicrobial use was more frequent at surgical wards (37.5%), followed by intensive care unit (21.3%), emergency unit (19.8%), clinical wards (16.1%) and pediatric (5.2%). After the first assessment, of 192 inpatients using antimicrobial drugs, 177 were not eligible to OPAT: 75% due to critical or instable clinical status, 13% with surgical antimicrobial prophylaxis and the remaining were pediatric patients or they had oral alternative therapy. Of 15 eligible patients, the multidisciplinary team assess clinical, social and logistic components for OPAT, including 7 (3.6%) cases with all OPAT criteria. Of these, two cases with osteomyelitis, two cases with urinary tract infection, two cases with catheter related blood infection and one with bacterial pneumonia. Multidrug-resistant microorganisms were isolated (ESBL Enterobacteriacea, meticilin resistant S. aureus, P. aeruginosa, E. faecium and E. faecalis). OPAT regimen proposed for these patients were vancomycin, meropenem or ertapenem, combined or isolated. CONCLUSIONS::  although few patients were identified for start OPAT modality this multidisciplinary strategy requires cost-effectiveness evaluation in different levels of the Brazilian health care system.