OBJECTIVES::  To assess health care resource utilization and associated costs, among Children Hospitalized with Respiratory syncytial virus infection in Rionegro, Antioquia, Colombia. METHODS::  Children with RSV infection admitted between January 2015 and December 2015 to Clinica Somer, Antioquia, Colombia, were included in this analysis. RSV-related health care resource utilization and direct cost was collected. We included only the direct medical costs (management cost, drug administration, supportive medications). Neither indirect costs nor direct non-medical costs were considered. All costs were adjusted to 2015 US dollars. (Currency rate: US$ 1.00 = COP$ 3,000). RESULTS::  RSV was detected in 116 of 261 hospitalized children with Bronchiolitis (44.4% CI 95% 38.5-50.5%), ages ranging between 2 months and 10 months (median: 6.59), and 58.8 % were males. The average length of hospital stay was 7.25 days (SD 6.53 days) and 13.8% were admitted to the ICU. Unadjusted annual RSV-related costs were US$ 59.501. Higher RSV-related costs were driven by greater mean annual RSV medication costs (US$22.802) followed by hospital stay (US$22.445) and the lower cost was by oxygen (US$1.072). The average annual direct costs per patient was US$512. CONCLUSIONS:: RSV infection causes significant disease burden in Rionegro, Colombia, leading to increasing health care resource utilization with high average annual direct costs per patient.