OBJECTIVES: Purpose of the study was to determine kinematic difference between overground and treadmill walking.  METHODS: A total of 30 healthy men, 30 healthy female aged 19-23 participated in the study. Inclusion criteria in the study were that participants no low back and lower extremity pain, no traumatic injury and foot deformities. Participants walked at their preferred velocity on overground. The treadmill velocity was adjusted average velocity obtained in overground walking. Walking in two conditions was captured by high speed camera and analyzed by motion analyses software. RESULTS:  In comparison between treadmill and overground walking was significantly different. Maximum hip flexion angle (P<0.017), maximum),maximum knee flexion (0.033)maximum ankle dorsiflexion (P<0.028) and maximum ankle planterflexion (P<0.044) were significantly different in the two conditions. In the male, maximum hip flexion (P<0.020), maximum knee flexion (P<0.019), maximum ankle dorsiflexion (P<0.018) ) were significantly different in the two conditions. For  female, maximum hip flexion (P<0.015), maximumankle dorsiflexion (P<0.045), maximum plan terflexion (P<0.021) were significantly different in the two conditions. Overground walking male of knee extension was associated with body mass (r=-411, p<0.05), hip flexion was associated with body height (r=0.433, p<0.05). Overground walking female of knee flexion was associated with body mass during (r=0.469, p<0.05), ankle dorsiflexion was associated with height (r=0.443, p<0.05). Treadmill walking in female, hip extension (r=-0.542, p<0.05), knee flexion (r=-0.342, p<0.05), ankle dorsiflexion (r=-0.469, p<0.05) was associated with body mass.Hip extension was associated with height (r=-0.542, p<0.05).  CONCLUSIONS: The study revealed significantly kinematic difference between overground and treadmill walking.