OBJECTIVES: assessment of health-related quality of life in a cohort of HIV-infected patients. METHODS: patients with diagnosis of HIV infection initiating antiretroviral therapy, receiving health education from a Pharmacist and a Doctor, belonging to the Health promoting entity “SURA”, in the  department of Antioquia (n=70). Evaluation period: Between August 2014 and February 2015. Measurements: Baseline (diagnosis), second measurement (one month after), third measurement (three months after). Questionnaire used: The World Health Organization Quality of Life (WHOQOL)-BREF was used after previous approval of the World Health Organization and SURA’s Scientific Direction. The Statistical software “Rstudio Version 0.98.1103– ©2009-2014 RStudio, Inc.” was used for that purpose. RESULTS: average age: 31 years [25-38], 92% men, 58% with higher education, 76% singles, 78% homosexuals, 74% employed. In general, the HRQOL behavior was: Baseline: 75 [63-90], 1st: 82 [70-88], 2nd: 83 [70-95]. When stratifying in terms of dimensions: Psychological: Baseline: 78 [63-85], 1st: 81 [70-86], 2nd: 81 [78-96]. Environmental: Baseline: 76 [64-82], 1st: 82 [68-88], 2nd: 82 [76-95]. Physical health: Baseline: 81 [62-86], 1st: 81 [70-81], 2^nd: 81 [70-86]. Relationships: Baseline: 69 [57-80], 1^st: 72 [57-80], 2^nd: 76 [57-100]. CONCLUSIONS: the most affected dimension measured by the WHOQOL at the beginning of antiretroviral therapy was “Relationships”. The Environmental and Psychological dimensions experienced greater improvement according to time.