OBJECTIVES: To evaluate the activities of immunization providers in terms of activities time and cost, and to calculate the immunization doses cost. METHODS: Time-motion and cost analysis study design was used. Five public health clinics in Mosul-Iraq participated in the study. Fifty (50) vaccine doses were required to estimate activities time and cost. Micro-costing method was used; time and cost data were collected for each immunization-related activity performed by the clinic staff. A stopwatch was used to measure the duration of activity interactions between the parents and clinic staff. The immunization service cost was calculated by multiplying the average salary/min by activity time per minute. RESULTS: The average time for child registration was 6.7 minutes per each immunization dose, and the physician spent more than 10 minutes per dose. Nurses needed more than five minutes to complete child vaccination. The total cost of immunization activities was 1.67 US$ per each immunization dose. Measles vaccine (fifth dose) has a lower price (0.42 US$) than all other immunization doses. CONCLUSIONS: The time spent on physicians’ activities was longer than that spent on registrars' and nurses’ activities. Physician total cost was higher than registrar cost and nurse cost.