OBJECTIVES: To evaluate the effect of aqueous extract of Boswellia serrata Roxb oleo gum on urinary electrolytes, pH and diuresis in normal albino rats. METHODS: Oleo gum weighing 500g was soaked in hot boiling water (1L) at room temperature for 3 days with occasional shaking. Filtrate was evaporated on rotary evaporator under reduced pressure (−760 mmHg) to a thick, semi-solid pasty mass of dark drown color that was completely solubilized in both distilled water and normal saline for in-vitro and in-vivo experimentation. Phytochemical analysis was carried out for alkaloids, saponins, anthraquinones, flavanoids and tannins. For in-vivo activity, five groups of with six animals in each were administered normal saline (10 ml/Kg, i.p.), Furosemide (10mg/kg) and crude extracts of Boswellia sarrata (10, 30 and 50mg/kg of body weight), respectively.  Toxicological effect of plant was undertaken in rats at a dose of 3000mg/kg. Data was expressed as mean ± SEM and analyzed using Graph Pad Prism (Graph PAD, San Diego, USA). Student t-test was applied for data analysis. p values <0.05 were considered significant. RESULTS: Phytochemical screening of Boswellia serrata Roxb confirmed the presence of saponins and flavonoids. Furosemide induced significant diuresis and electrolytes (Na+ & K+) excretion while plant extracts increased urinary output and electrolytes excretion in a dose-dependent manner. Diuretic index of test groups were1.36, 2.06 and 2.9, respectively while, Lipschitz value also confirmed diuretic activity in dose dependent manner. Urinary pH remained unchanged during the course of the study whereas, no lethality was observed at the dose of 3000mg/kg. CONCLUSIONS: Aqueous Boswellia serrata oleo gum extracts administered particularly at the dose of 50mg/kg significantly induced water and electrolytes with no signs of toxicity.