OBJECTIVES To evaluate the development level of Health Technology Assessment (HTA) in Latin-America and the Caribean (LAC), and the facilitators and barriers for international collaboration. METHODS A web-based auto-administered survey was designed by the International Network of Agencies for Health Technology Assessment (INAHTA) in collaboration with PAHO/WHO and mailed to 242 contacts in LAC. The survey evaluated: 1) Government/Institutional support; 2) Human resources; and 3) Facilitators and barriers for international collaboration. RESULTS Seventy-eight complete surveys from nine LAC countries were received (response rate 32%), 82% belonged to Argentina, Brazil, Chile, Colombia and Mexico. Government support to HTA activities was considered to be intermediate (31%) or low (53%) by the majority of the respondents, as well as the use of HTA evidence and the availability of resources to develop HTA. The application of HTA to public health decision-making was considered high or intermediate by 55%. Support from pharmaceutical industry was considered to be low by 61%. Lack of knowledge about INAHTA and the potential benefits of international collaboration, lack of support from international organizations as well as time and budget constraints were identified as the principal barriers to international collaboration. CONCLUSIONS Although the low response rate, the survey provided useful inputs about HTA in LAC. The perception of a majority of key informants is that HTA faces a low level of governmental support in most countries and limitations in budget and resources. Nevertheless, an opportunity of improvement is foreseen, given the apparent extended application of HTA in public health decisions. International collaboration efforts can focus on promotion of HTA and its benefits and should consider regional budgetary constraints.