OBJECTIVES: i. To assess the knowledge of Cervical Cancer, Human Papillomavirus and Human Papillomavirus Vaccine; the perception and intent to vaccinate among adult population in Mumbai, India. ii. To gauge physicians' attitudes towards HPV Vaccination and practice methods at their principal practice sites in Mumbai, India. METHODS:  A closed-ended question based survey was carried out among: i. Willing participants (n=327), 18 years of age or older. ii. Paediatricians, gynaecologists and general practitioners. RESULTS:  About 60% participants had a general understanding of HPV while 24% were familiar with the HPV vaccine. It was found that 3 out of every 5 doctors administer the vaccine, but only about 14% female participants had taken it. Due to lack of proper knowledge, 74% of the participants were apprehensive about taking the vaccine. Of the participants initially unaware, 40% were willing to take it after obtaining some information provided through the survey. CONCLUSIONS:  The utilization of the recently introduced HPV vaccine is challenged not only by lack of education, but also by the high cost, feasibility and logistics of the three-dose regimen. The need of the hour is to increase awareness about HPV as a major cause of cervical cancer in young and middle-aged women and the availability of this vaccine as an effective prophylactic method. With proper patient counselling and management strategies, healthcare professionals can play a vital role in increasing the acceptability of the vaccine in adolescents, parents and the community.