OBJECTIVES: To assess the female workforce and their job satisfaction in government hospitals in Quetta, Pakistan. METHODS: Mixed method Prospective and Retrospective study conducted on total of 579 female healthcare professionals in Government hospitals of Balochistan, Quetta from July-2015 to April-2016. All the female health care professionals (medical doctors, pharmacists, nurses) working in government hospitals targeted for the study. Study tool consists of Questionnaire for job satisfaction consists of domains i.e. Authority, Promotion, relation with patient, Relation with other health care provider, Pay and Professional development along with socio-demographic variables. All female employees present on their duties on time who agree to participate in study were included. Descriptive and inferential statistics have been done by using SPSS version 20. RESULTS: Majority of participants (n= 304, 52.5%) were nurses. having majority (n=155, 51.0) ranges between 25 - 30 years. Majority of them were married (n=184, 60.5%). Maximum respondents 97 (31.9%) were have experience of 1 – 5 years. Overall satisfaction score demonstrated that maximum respondents (n=500, 86.4%) were satisfied, association of mean satisfaction score with demographics was performed, none of demographics was significantly associated with overall satisfaction (i.e. p>0.05) except to job place (p=0.001) and Department (p=0.019). CONCLUSIONS: It is concluded that overall satisfaction was found to be satisfied, however it is showed that early career level in respondents were more satisfied, as the age level was increased there is low gradient of satisfaction with job.