OBJECTIVES:  Healthcare provider recommendation is critical to increase the human papillomavirus (HPV) vaccine uptake among the un-vaccinated adult population aged 18 to 26 years. Our aim was to estimate the effect of educational intervention tool in KAP score differences among adult respondents at three time points. METHODS:  The educational intervention consisted of an educational pamphlet or Fact sheet which contained information regarding HPV infection and HPV vaccination. The knowledge gained was assessed at baseline and post one to three weeks, whereas knowledge retained was assessed between baseline and post 3 to six months. McNemar test was used to compare knowledge scores between pre-test and post-test, and the proportion of correct responses for each question, respectively based on binomial distribution and Wilcoxon signed ranks test was used to estimate the precise differences in KAP scores between the three time points. RESULTS:  A total of 942 participants, responded to all the three phases of the study conducted in rural and urban areas. The pre-test and post-test KAP score differences shows a statistically significant difference in KAP scores (p < .001) for all the 16 knowledge based items. Participants who received the educational intervention had a significantly higher knowledge about HPV infection and HPV vaccination (p < .001). CONCLUSIONS: Educational intervention promotes an increase in knowledge about HPV and HPV vaccination. Further investigation should be conducted to determine if this intervention can be sustained beyond the short term and influence vaccination and pap screening behavior in women. Key: HPV infection, Knowledge, Attitude, Perception, Practice, HPV Vaccine, Educational intervention