OBJECTIVES: Stress is very common among university students. Social support and personality traits could change or buffer the effect of accumulative stress on students’ health. The objective of this study is to examine the relative association between psychosocial factors (perceived stress, perceived social support, and personality) and health status perception among West Virginia University (WVU) students. METHODS: An online survey was conducted with a structured questionnaire consisting of Perceived Stress Scale (PSS), Multidimensional Scale of Perceived Social Support (MSPSS), and Ten Item Personality Measure (TIPI), among WVU students between September 2015 and December 2015. Linear regression model was used to examine association between perceived health status and the psychosocial factors. RESULTS: A total of 207 participants met the inclusion criteria. The response rate was 161/207 (77.8%). The majority of our sample was females (68.9%), pursuing Bachelor's Degree (70.2%), and not married (91.3%). Mean age was 23.69±6.87 years. The average of the student’s perceived stress scores and perceived social support scores were 21.55± 6.91, and 5.13± 1.18 respectively. Better perceived health status was associated with less perceived stress (β=0.11, p<0.001), better perceived social support (β=-0.23, p=0.003), being more conscientious (β=-0.45, p=0.02), and emotional stability (β =0.37, p<0.001). Perceived stress was significantly negatively associated with perceived social support (p=0.003). CONCLUSIONS: Perceived stress, perceived social support and personality of the students affect their health status. Attention is required to highlight these factors on students’ health.