OBJECTIVES: The major objectives of our study was to investigate the prevalence rate of tuberculosis in our population. Bidirectional screening of established tuberculosis and diabetes patients for comorbid TB-DM individuals and effect of this comorbidity on outcomes of anti-Tuberculosis treatment.  METHODS: It was an exploratory, cross-sectional and retro-prospective study. This study took place at the outpatient DOTS clinic for TB, Tuberculosis & Chest Unit and Pharmacy Section at a secondary health care facility in Punjab, Pakistan. Retrospective study was performed by evaluating accessible previous data to check the trend either decreasing or increasing. Both diabetic and tuberculosis patients, consecutive registered during study, were screened for comorbidity and treatment outcomes comparison was done between TB-only and TB-DM groups. RESULTS: Annual upraise of 6% tuberculosis patients was noted in retrospective study. While prevalence of TB in established DM patients was 10.52% and prevalence of DM in tuberculosis patients was 6.89%. Cure rate, positive treatment outcome, in TB-only and TB-DM groups was 78% and 71% respectively.  CONCLUSIONS: We found annual increase in TB cases, while prevalence of TB and DM in established DM and TB population was higher than general population. There was consistency in the sputum smear microscopy and treatment duration among patients with TB‑DM and those with TB alone. So no negative effect on outcomes of anti-TB treatment was noted in our study. This highlight the need to promote awareness on nosocomial TB transmission in community and emphasized on bidirectional screening. The rising burden of DM may adversely affect TB control program. At present there is a need to have a strong collaboration between National TB and diabetes programs.