OBJECTIVES: To evaluate the importance of a health-educational interventional program to improve Health-Related Quality of Life (HRQoL) among Pulmonary Tuberculosis (PTB) patients in Pakistan, under the supervision of registered hospital pharmacists.  METHODS: A health-educational intervention to improve HRQoL was offered to the PTB patients through registered hospital pharmacists. In this non-clinical randomized controlled trial, PTB patients were briefed regarding treatment and management of PTB and their HRQoL was measured by WHOQOL-BREF. Both descriptive and inferential statistics were used to determine patients’ demographic characteristics and inter-group comparisons respectively. Data was analyzed by SPSS 21.0.  RESULTS: Two hundred and eighty PTB patients were randomly assigned for the study i.e. one hundred and forty patients in each group. No significant differences were observed in either group for mean age, gender, education level, occupation and income whereas a significant increase (p<0.001) in the WHOQOL-BREF score was observed in the interventional group.   CONCLUSIONS: HRQoL was significantly improved in the interventional group after the pharmacist-led interventional program which advocates the vital role of pharmacists in patients’ education and a better health care system of Pakistan.