OBJECTIVES: Uterine fibroids (UF) are associated with significant reduction in health-related quality of life (HRQL). This cross-sectional survey examined the impact of UF-related symptoms on HRQL using a disease-specific instrument, the UFS-QoL. METHODS: An online survey of US women between 18 and 54 years was conducted using 3 large respondent panels as a sampling frame. Data collected included demographics, UF prevalence, symptoms, HRQL and health-related productivity. Descriptive statistics were used to examine the impact of symptom presence, symptom severity, bothersomeness, and number of UF-related symptoms on multiple domains of HRQL. Analyses were weighted to match the population distribution of age, education, region, and household income of US female population. RESULTS: CONCLUSIONS: HRQL among women with UF was significantly impacted by UF-related symptoms. A more pronounced impact was observed as the number and severity of symptoms increased.