OBJECTIVES: Differences in vaccine schedules may result in additional costs beyond the differences due to pricing variation between two products. There are two oral rotavirus vaccines approved for use in Canada; one vaccine is approved as a two-dose schedule while the other is approved as a three-dose schedule. The objective of this study is to explore the cost of administration of a single dose of either rotavirus vaccine in each Canadian province as well as for the country overall. METHODS: Two extremes were considered: Every dose administered by a physician either (i) requires a visit solely for that purpose (cost = administration + visit), or (ii) occurs as part of a regular visit (cost = administration only). All costs were derived from the relevant fee for service agreement of each province and territory in Canada. Assumptions included 90% vaccine coverage across each province’s 2013 birth cohort and no significant changes since 2007 to the ratio of physicians to public health nurses (PHN) administering vaccines in each province.  Delivery by PHN was assumed to incur no cost. RESULTS: Scenarios (i) and (ii) above yielded administration costs of $6.4M and $600k, respectively, per vaccinated Canadian cohort.  Provinces with high percentage of physician delivery accounted for the majority of this cost in both Scenario (i) (NL: $128.6k, NB: $279.3k, NS: $312.1k, QC: $832.5k, ON: $3.8M, MB: $386.3k, BC: $544.1k) and Scenario (ii) (NB: $60.4k, NS: $114.2k, QC: $258.8k, MB: $85.1k, BC: $72.8k). CONCLUSIONS: Administration fees can be a costly factor in universal immunization schedules. As such, it is essential to account for the differences in approved administration schedule when evaluating vaccines during the assessment of publically funded program implementation.