OBJECTIVES: The main objective of this study is to assess the awareness regarding Hepatitis B among women in Abbottabad Pakistan. METHODS: A quantitative approach was used to gain the understanding. The study took place in two hospitals from April 2014 to June 2014. 317 patients were participated in the study. Patients aged 15 years and above, and familiar with Urdu, Hindko and Pashto (languages of Pakistan), and pregnant women were included in the study. RESULTS: A total of 370 questionnaires were distributed and 317 were received with a response rate of 84%. 95.3% of respondents were heard about hepatitis whereas 60.9% were unaware of its caused by virus. 63.1% knew about transmission of hepatitis via blood and blood product. Majority 80.5% of the respondents were aware of its transmission from mother to baby. 79.9% knew about transmission by un-sterilized syringes, needles and surgical instruments. Whereas 80.2% respondents were aware of its transmission by contaminated blood and blood products. CONCLUSIONS: The findings of this study in different age group indicate that Hepatitis B patients were unaware of the disease’s symptoms. Large percentage of patients knew about the transmission of the disease. Extensive health education campaigns should be provided to patients as well as in healthy population in both hospital and community settings. Physicians, pharmacists and nurses should play a role in developing a collaborative care model to provide education to the patients. Empowering the patients will be helpful in disease control as well as in the further spread of HBV to the healthy population.