OBJECTIVES: In 2012, the US Centers for Disease Control and Prevention published recommendations of one-time Hepatitis C virus (HCV) screening for adults born during 1945 through 1965. Evaluating U.S. HCV populations by birth segment may provide insights that could be increasingly relevant to payers and healthcare providers. METHODS: Unique respondent data from the U.S. National Health and Wellness Survey from 2009-2012 were analyzed. Individuals aged ≥18 years who self-reported a Hepatitis C diagnosis were stratified into 3 cohorts based on birth year:  pre-1946, 1946-1964, and post-1964. Characteristics, treatment rates, QOL (SF-12/36), and activity impairment (WPAI) were described. RESULTS: Individuals born between 1946-1964 represented 64.6% of respondents with Hepatitis C (13.0% were older; 22.3% younger). The 1946-1964 cohort had a higher proportion of males than the younger population (65.3% vs. 59.3%, respectively; p<0.05); 64.2% pre-1946 were male. Insured status was higher (p<0.05) in the older cohort (96.5%) versus the 1946-1964 (75.5%) or younger (70.2%) cohort. Reported current HCV treatment use was lower (p<0.05) in the older cohort (3.2%) versus 1946-1964 (10.7%) or post-1964 (21.4%). More than half in each cohort were treatment naïve (64.2% pre-1946; 53.5% 1946-1964; 53.4% post-1964). A lower (p<0.05) proportion (10.3%) of treatment naïve respondents born pre-1946 had a prior doctor recommendation for HCV therapy (versus 21.0% 1946-1964 or 21.1% post-1964). Mean percentage activity impairment was lower (p<0.05) among pre-1946 cohort (34.4%) versus 1946-1964 (45.3%) or post-1964 (45.1%). Mean Mental Summary Scores worsened from oldest to youngest cohorts (pre-1946=50.6; 1946-1964=43.8; post-1964=39.5). Mean Physical Summary Scores were higher for the younger cohort (43.6) versus 1946-1964 (40.2) or pre-1946 (41.2). CONCLUSIONS: In this Hepatitis C population analyzed by birth segment, individuals born 1946-1964 represented the largest segment of the population.  Results suggest that differences by birth cohort may exist within this population regarding their characteristics, treatment rates, and patient-reported outcomes.