OBJECTIVES: This study aims to evaluate safety culture dimensions among pharmacists using Hospital Survey on Patient Safety Culture (HSOPSC) developed by AHRQ. METHODS: We surveyed nationwide the situation of patient safety culture in 37 hospitals (18,960 persons) between 2011 and 2012FY, which were allowed for additional costs on patient safety countermeasures under the social insurance medical fee schedule in Japan.  RESULTS: We classified 37 hospitals into three groups by number of beds; A group’s bed number was 20 to 200 (six hospitals), B group’s was 201 to 400 (twelve hospitals), and C group’s was more than 401 (19 hospitals). The overall response rate was 87.9% (16,670/18,960 persons). Number of respondents in A group was 996 (1,116 persons; response rate: 89.2%) including 19 pharmacists, number of respondents in B group was 3,319 (3,674 persons; response rate: 90.3%) including 100 pharmacists, and number of respondents in C group was 12,355 (14,170 persons; response rate: 87.2%) including 340 pharmacists. The overall average positive response rate (RR) for the 12 patient safety dimensions of the HSOPSC was 46.6% in A group, 52.7% in B group and 51.0% in C group. In terms of occupational categories, RRs for pharmacists were 48.3% in A, 56.8% in B and 50.0% in C, RRs for physicians were 44.3% in A, 52.9% in B and 50.7% in C, and RRs for nurses were 43.4% in A, 52.8% in B and 51.6% in C, in each. RR for pharmacists was the highest among these three professionals in A and B groups. In terms of pharmacists, RRs in B group was the highest among three groups. CONCLUSIONS: The HSOPSC measurement provides the evidence for assessment of patient safety culture for pharmacists in Japan’s hospitals. This result suggested that pharmacists might be highly concerned with patient safety in Japan.