OBJECTIVES:  This study aims to investigate whether or not the frequency of internet use as well as the epidemiological and the demographic factors affect the assessment of general knowledge of health-related topics. METHODS:  Data from the FLASH EUROBAROMETER 404 were used for the purpose of this study. The sample size was 1000 individuals aged 15 years or over (n=1000). The dependent variable is the assessment of knowledge of health-related topics (four point scale, 1: very bad to 4: very good). Since the response variable is ordinal, Ordinal Logistic Regression was used during data analysis. Potential predictors in the model were the following: a) gender (1: male, 2: female); b) age; c) self-reported health status (1: very bad to 4: very good); d) existence of chronic health condition (0: no, 1: yes); e) frequency of internet use (1: never to 6: once a week or more often). RESULTS:  The Ordinal Logistic Regression Model indicates that frequency of internet use, gender and self-reported health status are significant. More specifically, we found that women have higher probability than men to assess their knowledge of health-related topics as good (OR=1.55; p=0.015). The same holds for healthier individuals (OR=1.42; p=0.036). In addition, individuals using more frequently the internet to search for health-related information have a higher probability of assessing their knowledge concerning health-related topics as good (OR=1.18; p=0.001) CONCLUSIONS: <span">