OBJECTIVES: To assess knowledge, attitude and perception of Polio (Poliomyelitis) among general public in Pakistan and to recommend suitable measures in order to improve the awareness of this perilous disease.  METHODS: A cross-sectional study was conducted on a newly developed, validated and structured self-administered research tool and data was collected by interviews. Face and content validity of the research tool was done by various healthcare individuals including academics, physicians, pharmacists and senior nurses. The research tool was also piloted on 15 healthcare providers in a different hospital before start of the study. A p-value < 0.05 was considered statistically significant. RESULTS: The findings of the study revealed that out of 1250 participants, 87.5% were males while 12.5% were females. 11.2% of the participants believed that vaccination was prohibited in religion, 85.4% participants were aware of Polio disease, and 84.5% believed that disease could be prevented by giving vaccines to children. 82.3% participants gave vaccine to their children whereas 55.2% knew the schedule of the vaccine for their children. This study also found that 88.8% of the participants wanted to eradicate disease from Pakistan.  CONCLUSIONS: The results of the study revealed that people have adequate knowledge about Poliomyelitis and wanted to eradicate it from Pakistan by participating in vaccination activities but still there are few who believe that Polio vaccine cannot prevent the disease.