OBJECTIVES: Few studies have estimated time and associated cost related to a paediatric vaccination visit. Time and Motion (T&M) methodology is suitable to collect efficiency-related outcomes. The aim was to quantify active healthcare professional (HCP) time and associated costs of a single vaccination process administered in a paediatric population in the UK. METHODS: Paediatric vaccination processes were observed in 6 General Practitioner (GP) surgeries across UK in children 2-14 months. Time to perform 7 clearly pre-specified tasks was recorded by trained observers using a stopwatch. Time of day measurements for subject time in GP surgery and vaccination room as well as consumables usage was collected. Time for tasks before vaccination day was obtained from opinion. A total 123 processes were observed (approximately 20 by site, equally distributed across visits 1 to 4). Analyses were run by site and pooled across sites using a random intercept model. Published 2014 PSSRU data were used to transfer HCP time into cost. RESULTS: Mean HCP time for a vaccination process (including a mean 2.75 vaccine doses) was 9.5min (95% CI: 7.7-11.3); site range: 7.0-11.3min. Associated cost (90% practice nurse, 10% support staff) was £6.2; range: £4.7-£7.7. Mean time for a single oral and intramuscular vaccine administration was 1.0 and 0.4min, respectively. Mean time for a single vaccine reconstitution was 0.8min. Mean time in GP surgery was 23.9min (95% CI: 19.2-28.5), of which 8.4min in vaccination room (95% CI: 5.5-11.2). When including average supplies cost (£0.27), as well as an estimated 10.1 minutes per vaccination process prior to vaccination day (£5.5), total estimated cost per visit was £12.0. CONCLUSIONS: Paediatric vaccination requires substantial resources, including staff, facility, and parental time. Potential changes in the vaccination schedule would have an impact on associated time and cost and the vaccination unit’s capacity to managing subjects.