OBJECTIVES: The number of patients in the Czech Republic treated for diabetes in 2013 was nearly 862 thousand. The Type 2 diabetes mellitus share was 91.7 %. The number of treated diabetics has been increasing in a long-term trend as well as the number of their chronic complications. The goal of the study is to estimate the cost of type 2 diabetes from the health insurance perspective in a newly established regional diabetology outpatient facility. METHODS: The cost-of-illness analysis of the direct costs was performed from the third-party payer perspective based on data from the year 2014. The bottom-up approach was used. Data of all 552 patients with Type 2 diabetes were (all patients with Type 2 Diabetes in the ambulance) were taken into consideration. Altogether 1 696 consultations were provided to the patients during the particular year. RESULTS: The average cost of consultations for one type-2-diabetes patient per year was CZK 840.56 (~EUR 30.5), the average cost of treatment for one patient was CZK 10 336 (~EUR 375), the average material cost was CZK 2 835 (~EUR 103) per patient, and CZK 113.7 (~EUR 4) was the average cost for amputation per patient, and the average cost of diabetic nephropathy was CZK 41 841 (~EUR 1 518) per one patient. The total average cost per one type-2-diabetes patient was CZK 55 956 (~EUR 2.031) in 2014. CONCLUSIONS: Our data indicate that the cost of type 2 diabetes mellitus was CZK 55 956 (~EUR 2 031). A longitudinal study is, however, required to confirm the development of the average patient cost and take account of the cost of complications.