OBJECTIVES Evaluate validity and reliability of the UISESS-B oral health questionnaire in a Mexican diabetic, systemic hypertension and obese patients in the primary health care level. METHODS Were studied 105 subjects with diabetes, systemic hypertension and/or over-weigh-obesity in the context of a diabetic’s control group in the Instituto Mexicano del Seguro Social. Was applied an oral health questionnaire named UISESS-B previously validated. The instrument is divided in three main areas: oral health care habits, oral health and nutrition related habits, and oral health care related symptoms and signs. RESULTS Construct validity was confirmed by the correct compilation of the items included in the two main constructs: habits and perceptions each one including specific multi-item questionnaires (correlation coefficients ranged from 0.30 to 0.80, P˃0.001); the reliability was confirmed by the internal consistency (Cronbach alpha: 0.55 for the construct habits and 0.71 for the construct perceptions). CONCLUSIONS The UISESS-B questionnaire shows significant validity and reliability, suggesting its use as an instrument useful to be used as a patient reported outcome in oral health in patients with chronic diseases.