OBJECTIVES The greatest burden of the HIV infection is felt in sub-Saharan Africa. Nigeria is rated among the first three largest countries with HIV population. Pharmacists play an important role in the care of HIV patients especially by providing pharmaceutical care. Thus it is imperative to assess the experience and satisfaction of HIV patients with the pharmaceutical services they receive as this will ensure that quality care is rendered. The objective of this study was to assess HIV-infected patients’ satisfaction with pharmacy services provided in South-Eastern Nigeria’s HIV/AIDS clinics. A secondary aim was to repeat the validation process in order to confirm the initial validation of PSPS questionnaire.  METHODS The survey was conducted in 6 HIV/AIDS clinics in South-Eastern Nigeria. Multi-stage sampling technique was used to select the clinics. Re-validation techniques employed include factor analysis, reliability test, and construct validity. The level of satisfaction of the participants was determined. Variance in satisfaction score among the different demographic variables was also assessed. RESULTS The response rate was 81.85%. The Cronbach alpha value was 0.84. The 16 items of PSPS questionnaire resolved into 4 factors as established by the initial validation exercise. Re-validation of the PSPS Questionnaire’s construct revealed the same result obtained in the original validation as items that were related showed convergence while items that were not related showed divergence. With the exception of item 12, the average score obtained in the survey was high. Most of the average scores ranged from 4 to 5, signifying that responses ranged from agree to strongly agree. CONCLUSIONS HIV patients were highly satisfied with services rendered by the pharmacists in South-Eastern HIV clinics in Nigeria and PSPS proved to be a reliable and valid instrument for measuring satisfaction of HIV patients with pharmacy services.