OBJECTIVES: To assess the job satisfaction of the nursing staff of the Pafos General Hospital in Cyprus. METHODS: A cross-sectional survey was conducted in April 2014. All 150 nurses (RR=100%) working at the General Hospital of Pafos filled out a standardized, self-administered questionnaire with a 5-point Likert scale, regarding the assessment of job satisfaction, working conditions and personal well being. The questionnaire scoring from 1 represented “very dissatisfied” to 5 “very satisfied”.  In order to investigate the association among job satisfaction as dependent variable and sociodemographic characteristics of the sample, satisfaction of working conditions and personal well being as independent variables, multiple linear regression analysis was performed with backward method. Also, correlation analysis, using Spearman rank coefficient, was conducted to quantify the strength of association among job satisfaction and independent variables. RESULTS: The majority of the sample (64.7%) was female and permanent employees (60.7%). The mean age was 33.7 (S.D.9.7) years. The personal well being score was 4.1 (S.D.0.6), which means satisfied. The working conditions and job satisfaction scores were 2.2 (S.D. 0.7) and 2.9 (S.D. 0.9), showing low and moderate satisfaction respectively. Age (β = -0.321, 95% C.I. -0.650, -0.008), permanency of job (β = 0.346, 95% C.I. -0.005, 0.697), satisfaction of working conditions (β =0.532, 95% C.I. 0.331, 0.732) and personal well being (β = 0.444, 95% C.I. 0.188, 0.700) were significant predictors of overall satisfaction (p≤0.05). Positive correlation was found between job satisfaction and working conditions (r_s =0.349, p ≤0.001) and personal well being (r_s =0.185, p ≤0.024) respectively. CONCLUSIONS: The assessment of nurses’ working conditions appears to contribute to the improvement of job satisfaction, the enhancement of productivity and nursing care outcomes.