OBJECTIVES: This study evaluated knowledge and attitude towards human papilloma virus (HPV) vaccination among women ages 18-24 years and examined predictors of attitude towards HPV vaccination.  METHODS: A cross-sectional, self-administered questionnaire was delivered to a convenience sample of female University students in 2009. Questions on knowledge (9 items) and attitude (2 items) were measured using a 5-point Likert scale. The instrument also included questions on demographics, HPV vaccination status, source of HPV-related information, and healthcare professional’s recommendation. For the purpose of modeling, knowledge (yes/no) and attitude (positive/negative) was reduced to a binary variable. Data was coded and analyzed using SAS v9.2, by conducting descriptive analyses, chi-square tests and logistic regression. RESULTS: A total of 136 subjects completed the survey with a response rate of 46.5%. The mean age of the sample was 20.67(±1.78) years with the majority being unmarried (88.97%) and insured (72.06%). HPV vaccination rate was 11%. The mean summary knowledge score was 4.4 (±3.0) with a reliability coefficient of 0.7. The mean summary attitude score was 2.7 (±1.2) with a reliability coefficient of 0.8. In the logistic regression model, health care professional’s recommendation to administer HPV vaccine was a significant predictor of positive attitude towards the vaccine (OR=2.886, 95% CI=1.186-7.020, p<0.05) after controlling for race, education, age, knowledge, and vaccination status. CONCLUSIONS: A healthcare professional’s recommendation to consider the HPV vaccine was a significant predictor of attitude towards the vaccine. Suboptimal knowledge and negative attitude towards HPV vaccination underscore the need for active education and accurate dissemination of information to women regarding HPV vaccination.