OBJECTIVES: Increased life expectancy of HIV-infected individuals has lead to an increase in number of uninfected children living with their HIV-infected parent(s). This study investigated the healthcare utilization and health status of these uninfected children. METHODS: In a cross-sectional matched case-control analysis, healthcare utilization and health status were compared between uninfected children (2 yrs-15 yrs) currently residing with- HIV-infected parent(s) (parent group) and HIV-negative parents (comparison group). HIV-infected parents provided information on the parent group (face-to-face interview). A representative sample from MEPS-2006 database provided information on the comparison group. HIV-infected parent were those with a prior diagnosis of HIV/AIDS (ICD-9-CM 042, 043, V08). Each child from the parent group was matched with two children in the comparison group (1:2 ratio) using propensity score technique. Multivariable logistic and negative-binomial regressions were conducted to control for patient-related and parent-related characteristics. RESULTS: Upon matching, 89 children living with HIV-infected parent(s) matched with 178 children of HIV uninfected parents. Both groups were similar in their demographic and socio-economic characteristics including parents’ health insurance status. Children of HIV-infected parent(s) were 1.682 times (95% CI: 1.115-6.453) more likely to have a doctor visit and had 49% (p=0.0206) more doctor visits than comparative group. The parent group had 3.0 times more hospital visits (p=0.0244) and 86% more emergency room visits (p=0.0464) compared to comparison group. Though the parent group showed no difference in their school/daycare absenteeism, they were 4.041 times more likely (95% CI: 1.887-13.471) to be overweight and 5.520 times more likely to be on a psychotropic medication (95% CI: 1.503-20.276) compared to comparison group. CONCLUSIONS: This study found children living with their HIV infected parent(s) had higher healthcare utilization and compromised health status, especially mental health, compared to those living with HIV-negative parents. Long term impacts of the study findings needs to be further investigated.