OBJECTIVES: To carry out the cultural adaptation and calibration in Spain of the universal Spanish version of two item banks previously developed and translated by the PROMIS Group: “Pain-Behavior-PB” (39 items) and “Pain-Interference-PI” (41 items). This will allow construction of two Computerized Adaptive Tests (CATs) for evaluating Health-Related Quality of Life (HRQoL) in patients with pain. METHODS: Forward and backward translations of PB and PI items were carried out. The alternative translation was then compared to the existing PROMIS translation and reviewed by 25 patients and 6 experts to evaluate the relevance and comprehensibility of items. Recommendations to revise the universal Spanish were evaluated by the PROMIS Statistical Center and by a linguist from Spain. The revised items were cognitively debriefed with 5 patients following the PROMIS Interview Script and discrepancies were resolved. The revised universal Spanish version of both item banks was administered to a sample of 236 subjects with chronic pain of any etiology. Unidimensionality and local independence were evaluated. The calibration of the items was done using the Samejima’s graded response model. RESULTS: The process of cultural adaptation of both item banks for use in Spain resulted in the amendment of 8 items (5 PB -3 PI). Unidimensionality and local independence of items of both banks were confirmed. Discrimination parameters ranged from moderate to very high in almost all items of both banks. Differences according to gender were not statistically significant in PB bank (F(1,225)=3.24;p=0.073) nor in PI bank (F(1,224)=2.072;p=0.151). Despite the relatively small sample size, the standard errors for the item parameters are within an acceptable range (<1),with the exception of three extreme threshold parameters. CONCLUSIONS: The universal Spanish PROMIS Pain Behavior and Pain Interference have been calibrated in Spain and two CATs have been built to evaluate HRQoL of patients with pain in daily clinical practice.