OBJECTIVES Mononucleosis is a non-fatal disease that is most widely seen in the adolescent ages of teenagers and toddlers. Mono is caused by the Epstein-Barr virus; a member of the herpes virus family and one of the most common human viruses, affecting as many as 95% of adults between 35 and 40 years of age. When infection with EBV occurs during adolescence or young adulthood, it causes mononucleosis 35% to 50% of the time. Our objective is to use data from the National Inpatient Sample for 2005, conducted by the Agency for Healthcare Research and Quality to explain these and other correlations with respect to mononucleosis. METHODS Within the sample, there were 2000 observations of patients with mono and 1814 without mono. The SAS statistical analysis software was used to produce table, graphical, and regression analysis to explain data correlations and discrepancies among the diseased population and the non-diseased population or the control group. RESULTS Demographic variables of gender and race were normally distributed within the disease group. Procedures of injection of antibiotic, venous catheterization, biopsy of bone marrow, tonsillectomy with adenoidectmy, along with diagnosis of disorder of fluid, acute tonsillitis, acute pharyngitis, general symptoms, peritonsillar abscess, and other symptoms involving abdomen and pelvis were seen as predictive variables correlated with having the disease. Because a wide array of procedures were seen in the disease population, the most common procedure classifications were extracted and used to perform regression analysis. CONCLUSIONS Procedure classes of other operations on lung and bronchus, incision, excision, and anastonmosis of intestine, along with incision, excision, and occlusion of vessels, and operations on bone marrow and spleen were found to be the best indicators of predicting length of stay and total charges of the diseased group.