OBJECTIVES: Apply and further validate the Patient Satisfaction with Pharmaceutical Care Questionnaire (PSPCQ) in a different pharmacy practice setting. METHODS: Five hundred questionnaires were distributed to patients dropping off a prescription at a high-volume (500 prescriptions/day), 24-hour chain pharmacy, during a 2-week period. PSPCQ is a self-administered questionnaire and comprises of 20 Likert-type items that measure patient satisfaction with functional and technical dimensions of pharmacy services, which are represented by Friendly Explanation (FE) and managing therapy” (MT) subscales. Patient satisfaction and psychometric properties of PSPCQ were evaluated using SPSS. Pearson's correlation and Student's t-test were used. RESULTS: Survey response rate was 24.2% (n=121). Chronbach's alpha for FE and MT scales were 0.979 and 0.980, respectively. Mean scale scores for Global, FE, and MT were 2.88 (SD=1.28), 2.98 (SD=1.27), and 2.74 (SD=1.35), respectively. Mean differences in mean scores were 0.24 (P<0.001) between FE and MT scales, -0.097 (P<0.001) between Global and FE scales, and 0.15 (P<0.001) between Global and MT scales. Inter-scale correlation coefficients were 0.938 (P<0.001) for FE and MT, 0.988 (P<0.001) for Global and FE, and 0.979 (P<0.001) for Global and MT. “How well the pharmacist answers your questions” received second highest rating of 3.21, while “Promptness of prescription drug service” received the lowest rating of 2.58, out of the twenty items. CONCLUSIONS: Significantly higher (P<0.001) overall mean score on MT scale compared to FE scale suggests that patients distinguish between activities related to pharmaceutical care (MT) and friendly service (FE); and, since usually higher scores are expected from patients on familiar items, construct validity was demonstrated. Practicing pharmacists, in this setting, supported face validity. PSPCQ demonstrated external validity, in a high-volume retail pharmacy setting. PSPCQ can be used to assess patient-reported quality, in addition to patient awareness, of pharmaceutical care in the community pharmacy setting.