OBJECTIVES: To estimate the healthcare resource utilization and their associated costs secondary to fasciectomy of Dupuytren´s disease treated according with usual medical practice in public hospital centers in Spain. METHODS: This multicenter, observational, retrospective cohort study, extracted data through the revision of medical records of three tertiary public hospitals. Each center should recruit 40 patients which were operated for Dupuytren´s disease, as principal diagnose of  Minimum Data Set, in which the surgical procedure conducted was fasciectomy, during 2007-2009. To collect all the resources consumed during surgery, a healthcare resource utilization form was designed. Demographic (age, gender, occupational status), clinical (time of evolution of the pathology and comorbilities) and healthcare utilization (hospitalizations, medical visits, test, and drugs) data were collected under medical routine. Unitary costs were provided by e-SALUD and BOT data base. RESULTS: A total of 123 subjects (86.2% men; 35.8% active workers) were identified. 17.8% of subjects were diagnose of Dupuytren before year 2000; 8.4% between 2000-2005 and 73.8% after 2006. 81.3% of patients had at least one comorbidity, being hypertension (45%) the most frequent. 71.6% of patients were hospitalized in orthopedist (75%) and plastic surgery unit. Mean(SD) length of hospital stay was 1.5(1.1) days. 28.4% there were operated in ambulatory surgery. All the patients had follow-up visits after surgery, 27% needed physical therapy, 88% performed preoperative tests and 8% visit the emergency room after surgery. Healthcare mean costs were as follows: fasciectomy €1074(0); hospitalizations €978(743); ambulatory €186(10); follow-up visits €260(173); emergency rooms €13(53); tests €132(121); drugs €7(9); physical therapy €46(134). Total cost for patients with Dupuytren’s disease treated with surgery was €2304(825). There were no significant differences between the three centers analyzed; p=0.181. CONCLUSIONS: This evaluation suggest that healthcare resources utilization for surgical treatment for patients with Dupuytren’s disease may cost €2,304(825) per surgery (fasciectomy) treated under usual medical practice in Spain.