OBJECTIVES: The main objective of this study was to evaluate the utilisation of antineoplastic agents in Slovak Republic during the period of 2006-2010. METHODS: Statistical analysed data including the number of medicine packages, DDD and financial expenditures were abstracted from the Slovak Institute of Drug Control. Key data were provided by wholesalers due to their legal obligation towards the SIDC. RESULTS: Consumption of antineoplastic agents in terms of DID (DDD/1000 inhabitants/day) reached its highest peak in 2007 with 31,12 and the lowest value of DID was observed in 2009 with 27,30. The total expenditures doubled their volume within period of 2006-2010 from 56,021,412 € to 111,646,240 € respectively. Number of delivered packages showed slight increase from 426,412 in 2009 to 629,782 in 2010 while price per single package was rising from 131,29 € (2006) to 197,68 € (2008) and then decreased to 177,28 € (2010). Resulting from further study the highest consumption in terms of DID was reached by gemcitabine (7,38 in 2006 and 7,21 in 2010), ifosfamide (5,91 in 2006 and 6,94 in 2010) and fourouracil (2,56 in 2006 and 3,26 in 2010). Expressed in financial units the most costly antineoplastic agent in 2006 was imatinibum with 8 569 021 €, followed by rituximab with 4,896,000 € and irinotecan with 4,888,660 €. In 2010 reached paramount financial consumption bevacizumab with 17,771,426 €, trastuzumab with 10,173,699 € and imatinibum with 8,212,353 €. CONCLUSIONS: Expenditures for antineoplastic agents are continually rising as a result of biological treatment establishment. There is observed significant increase of their consuption due rheumatic diseases treatment.