OBJECTIVES: The aim of this study was to determine the reliability and validity of the Fatigue Symptom Inventory (FSI) for the Turkish population. METHODS: The research was conducted in the university students. The study sample included 200 student, (56% women, 44% men) Data were collected with a sociodemographic form, FSI, SF-36. The original instrument was translated and back translated by two independent translators. For psychometric measures, a small sample was used to check the initial comprehension and factibility. Cronbach’s Alfa was used to assess reliability and factor analysis to assess dimensionality. The SF 36 were used for concurrent validity. RESULTS: The internal consistency coefficient (Cronbach's alpha) of FSI was 0.91. Factor analysis of the scale revealed that it was composed of 3 factors with Eigenvalues >1.0, accounting for 65.4% of the total variance. All items of the Turkish FSI had a factor load ranging from 0.286 to 0.820 and they all belonged to 3 factors. There was a strong relationship between FSI, and SF 36 (r= between 0.651-0.759) . CONCLUSIONS: The research suggests that the validity and reliability of the Turkish FSI are satisfactory, and that it can be used in Turkey.