OBJECTIVES: Hospital acquired infections, also called as nosocomial infections, are among the major causes of death and increased morbidity among hospitalised patients. This study assessed the validity of microbial control or cleanliness procedure of a public hospital in the Punjab state of India and reported the type of microorganisms and their count that leads to the outburst of various respiratory tract infections, urinary tract infections, blood stream infections, surgical site infections, neonatal sepsis and many more. This study proposes the concept of implementation of quality assurance department(for line clearance) in hospital setting. METHODS: The dust samples for microbial check was taken, using sterile swab sticks, from beds, floor, walls, usable items or other surgical equipment of claimed to be clean operation theatre, labour room and gynaecology ward. All the materials used while collecting samples including culture-media were pre-sterilised. Samples were tested in a pathology lab for microbial analysis. Final results were received from laboratory on fifth day after the sample was taken. RESULTS: The results were found to be very alarming as different microorganisms with cell count tremendously high as compared to the permissible limits were reported. The microorganisms reported were Bravibacillus brevis, Bacillus cereus, Aerococcus viridans, Bacillus jeotgali, Pseudomonas monteilli and Stenotrophomonas maltophilia. Infections caused by these microorganisms are life threatening for people of every age particularly newborns and immuno-deficits. CONCLUSION: This study offers the concept of line clearance process in hospitals as a preventive measure for hospital acquired infections. This process will involve validation of the infection or microbial control process and seek quality assurance approval before allotment of rooms to patients including operation theatres and intensive care units. This may reduce the nosocomial infections to a greater extent keeping in mind that prevention is better than cure and a moderate rate rise is better sooner than later.