OBJECTIVES: To develop a scale of Nutrition Related Quality of Life (NRQoL) in healthy children of 6 to 12 years old to be used in nutritional interventional studies in Spain. METHODS: The process of development of the DANN scale (Escala DAnone de calidad de vida relacionada con la Nutrición en Niños/as) included a literature review to define potential dimensions, a qualitative research phase for the elicitation of concepts including interviews to clinicians, psychologists, Focus Group with the target population and expert meetings for the item generation (version one). The face and content validity was analyzed by means of a cognitive debriefing process (version two). The quantitative research phase consisted of an evaluation of the scale in 125 children followed by a standard item reduction (version 3). This new version was administered to 500 children from different sites in Spain to evaluate the psychometric properties (reliability, construct-related and content-related validity) of the final version. RESULTS: The internal consistency of the DANN self-administered scale appears satisfactory (Cronbach’s alpha= 0.78). The underlying dimension structure of the scale was conducted by a principal component analysis with varimax rotation. The three first dimensions explained 42% of the total variance and conceptually corresponded to the dimensions initially defined (construct-related validity). The first dimension gathers the attitudes and beliefs related with healthy nutrition, consisting of ten items (23%). The second dimension is related to the physical well-being and includes six items (11%). The third dimension is related to the psysich well-being and contains three items (8%). Two different versions of the scale were developed according to the age of the children. CONCLUSIONS: The DANN scale is the first questionnaire of NRQoL developed in Europe and can be a useful tool for the evaluation of the effects of a nutritional intervention in healthy children.