Cytotoxic and antiviral potential of Moxidectin against influenza virus H9 was evaluated. The cytotoxicity of moxidectin was evaluated on primary fibroblast cell line through MTT assay. Antiviral activity in vitro and in vivo was tested on 9-days old chick embryo. Cytotoxicity and antiviral activity of Moxidectin is high at first two conc. 200µg/ml and 100µg/ml while safe at lower conc. 50, 25, 12.5 and 6.25µg/ml. It was concluded that concentrations of moxidectin 200 and 100µg/ml were cytotoxic and lower concentrations were safe. Moxidectin showed strong antiviral activity against influenza virus H9 at all concentrations but found to be safer on concentrations like 50, 25, 12.5 and 6.25µg/ml.