OBJECTIVES: This study was conducted to evaluate the effect of a pharmacist initiated health education programme for improving Hepatitis-B patients' disease state knowledge, attitude, practice and health related quality of life in Quetta city, Pakistan METHODS: The study was conducted as non-clinical randomization control trial. It was divided into four phases: pre-interventional assessment, training of hospital pharmacists, development and implementation of the intervention program and post-interventional analysis. The pre-interventional phase analysed the Hepatitis-B (HB) patients’ knowledge, attitude and practice and current status of Health Related Quality of Life (HRQoL).  RESULTS: Three hundred and ninety HB patients were targeted for the study. The pre-interventional analysis revealed poor knowledge (mean score 8.48 ±2.7 out of 20), negative attitude (mean scores 3.87±1.2 out of 7) and bad practice (mean scores 2.37±1.0 out of 8). The HB patients also had poor Health Related Quality of Life (mean score of 37.22±30.0 out of 100). The post-interventional data were available from 126 patients of interventional group and 151 patients of control group, giving a response rate of 64.6% and 77.4% respectively. No significant association was observed among demographics variables. However, knowledge, attitude and practice, and HRQoL scores were significantly associated (p<0.001) when compared between interventional and control group after the completion of the intervention. There was an increase in mean knowledge score (15.46±2.2), attitude score (5.05±1.1), practice (5.98±1.1) Health Related Quality of Life (48.16±25.2). The inter-group difference among pre- and post- interventional groups reported a significant difference (p<0.001) when knowledge, attitude and practice and HRQoL were compared.  CONCLUSIONS: The educational intervention significantly increase in the HB patients' knowledge, attitude and practice, and HRQoL. Therefore, the role of pharmacists in patient education must be formalized and acknowledged as an official part of the healthcare system.