OBJECTIVES: The study was conducted to detect and evaluate the medication errors in different hospitals in province of the Punjab, Pakistan. METHODS: A retrospective study was designed involving different hospitals namely: Shaukat Khanum Memorial Cancer Hospital Lahore, Mayo Teaching Hospital Lahore, Allied Teaching Hospital Faisalabad, District Head Quarter Hospital Sargodha, and District Head Quarter Hospital Gujrat of province of the Punjab, Pakistan. Medication errors were detected from the records of the patients on period from October to December 2010. Errors were categorized into Prescription error, Dispensing Error and administration Error. Descriptive statistics were used to describe demographic and disease characteristics of the patients. Percentages and frequencies were used to present the data.  RESULTS: A total of 5972 (SKMCHL), 7950 (ATHF), 8249 (DHQS) and 6325 (DHQG) were registered. A sample of 4500 prescription from each of the hospital was taken for the study. A total of 50 (1.1%) of errors were detected form the record of Shaukat Khanum Memorial Cancer Hospital Lahore, in which administration errors were the highest (n=18). For Mayo Teaching Hospital Lahore there were 169 total errors were detected, in which prescription errors were the highest (n=60). For Allied Teaching Hospital Faisalabad there were 186 total errors were detected, in which administration errors were the highest (n=68). For District Head Quarter Hospital Sargodha there were 252 total errors were detected, in which administration errors were the highest (n=92), and District Head Quarter Hospital Gujrat there were 266 total errors were detected, in which administration errors were the highest (n=96). CONCLUSIONS: There were minimum numbers of medication errors observed in different hospitals, but the frequency of medication errors in private hospitals were lower then governmental hospitals. The roles of Pharmacists are needed to be enhanced so that these minimal errors should also be avoided.