OBJECTIVES: To assess the knowledge and practice with respect to use of medicines, among post-graduate pharmacy students. METHODS: This cross-sectional study was carried out among the post-graduate students of NIPER, Mohali, during the period of Nov. 2014 to Feb. 2015. The method of data collection was based on pre-designed questionnaire containing close ended questions. Participants were randomly selected. Questionnaire inquired about their knowledge with respect to medicine, attitude and practising behaviour. Reponses received during the study period were coded and analysed using SPSS. RESULTS: A total of 200 students responded to the study. All the respondents were postgraduates in the age group of 20-30 years. About 95% respondents completed their dosage regimen as prescribed. Almost all the respondents (86%) said that they always shake the cough syrup bottle before use. Almost a three-quarter respondents asked the prescriber or dispenser about the possible side-effects of the prescribed medication. Majority (94%) of the respondents practised self-medication. Most common reasons for practising self-medication was found to be convenience (37.5%) followed by quick relief (25%), time saving (15%), economical (8.5%) and others (14%). 71% of the respondents reported the habits of sharing medicine with others. High prevalence (91%), of unused medication was reported. Most common reason cited for unused medication was discontinuation of medicines when patients start feeling better. CONCLUSIONS: The result of this study reflects a high prevalence rate of self-medication and unused medication. This needs to be addressed appropriately. Promotion of rational use of medicine is important to achieve the required effectiveness of the medicines.