OBJECTIVES: The study intended to assess the impact of educational intervention on knowledge of osteoporosis among female university students of Quetta. METHODS: This interventional study was conducted on female university students by using convenience sampling technique. A total of 163 female students were enrolled for the study, these are those female students who did not heard about the disease named as osteoporosis. These subjects were provided with a self-explanatory brochure that contain basic information regarding osteoporosis. The intervention was completely theoretical in nature. After two days interval participants were contacted again and asked to complete a  pre-validated questionnaire containing 20 questions related to osteoporosis knowledge. Descriptive analysis was used to demonstrate the demographic characteristics of the study population. Inferential statistics (Mann-Whitney U test and Kruskal Wallis tests and Wilcoxon mean rank test, p< 0.05) were used to assess the significance among study variables and to assess the impact of educational intervention on knowledge. RESULTS:  Average score of knowledge was 14.18±2.7 (20 max). The educational intervention had a significant effect on knowledge scores of the respondents (Wilcoxon rank test p< 0.005) (considering the pre-intervention knowledge score as zero). Certain demographic characteristics (academic degree and living status) does affect knowledge scores of the study respondents. CONCLUSIONS: Although adequate improvement of osteoporosis knowledge scores were reported after educational intervention, yet efforts should be made to bring change in the attitudes and practices of the female student by the help of intensive educational programs based on specified behavioral learning theories for better disease knowledge and prevention.