OBJECTIVES: To evaluate Knowledge, Attitude and Practices (KAP) of Hepatitis-B (HB) in general population of Quetta city, Pakistan METHODS: A cross sectional, descriptive study was undertaken with 1000 healthy individuals (aged 18 years and above) from April 2011 to July 2011. KAP regarding HB was assessed by using a pre validated questionnaire (20 questions for knowledge, 7 for attitude and 8 for practices). Descriptive analysis was used for elaborating participants’ demographic characteristics. Mean scores were taken into account for the assessment of KAP. Spearman’s rho correlation was used to identify the association between KAP. All analyses were performed using SPSS 16.0 RESULTS: A total of 1000 questionnaires were distributed, out of which 843 were returned (response rate of 84.3%). Seven hundred and eighty were included into the final analysis (sixty three questionnaires were exclude because of incomplete data). Four hundred twenty (53.8%) were male, with mean age of 32.76±9.40 year. Two hundred eight (26.7%) had intermediate level of education and 354 (45.4%) were unemployed. Majority (n=126, 16.2%) had monthly income of Pk.Rs. 5001 to 10,000, and 418 (53.6%) had urban residency. Mean scores for knowledge, attitude and practice were 8.74±2.7, 3.72±1.2 and 2.76±1.1 respectively. A significant yet moderate positive linear correlations between knowledge-attitude (r=0.296, p<0.01) knowledge-practice (r=0.324, p<0.01) and attitude-practice (r=0.331, p<0.01) were observed.    CONCLUSIONS: Results of the present study show that knowledge, attitude and practice of healthy general population regarding hepatitis B are low. There are, however, positive linear correlations between knowledge-attitude, knowledge-practice and attitude-practice. This reaffirms that better knowledge can lead to positive attitude resulting in good practices. This can prevent the healthy individual from acquiring HB infection.