OBJECTIVES: To evaluate Knowledge, Attitude and Practice (KAP) of Hepatitis-B (HB) patients in Quetta city, Pakistan METHODS: A cross sectional, descriptive study was undertaken with 390 HB patients attending two public hospitals of Quetta city, Pakistan. KAP regarding HB was assessed by using a pre validated questionnaire (20 questions for knowledge, 7 for attitude and 8 for practices). Descriptive analysis was used for elaborating patients’ demographic characteristics. Mean scores were taken into account for the assessment of KAP. Spearman’s rho correlation was used to identify the association between KAP. All analyses were performed using SPSS 16.0 RESULTS: Two hundred and thirty two (59.5%) were males with majority (n=136, 34.9%) falling in age group of 38-47 years. Mean age of the patients was 36.21±9.2 years. One hundred and forty (26.7%) had primary level of education and 110 (28.2%) were working in private sector. Eighty Three (21.3%) had monthly income of in between 10001-15000 Pakistan rupees with 273 (70%) having urban residency. The mean scores for knowledge, attitude and practice 8.48±2.7 were 4.07±1.2 and 2.37±1.1 respectively. A significant linear correlations between knowledge-attitude (r=0.221, p<0.01) knowledge-practice (r=0.466, p<0.01) and attitude-practice (r=0.224, p<0.01) were observed.   CONCLUSIONS: Results of the present study show that KAP among HB patients is at lower side. Positive linear correlations, however, predicts that better knowledge can leads to positive attitude and good practices which will prevent the further spread of infection from patients to healthy population.